Marvel Legends Hulkling and Wiccan

Marvel Legends Ultimate Spider-Woman

Marvel Legends Scourge of the Underworld (Dennis Dunphy)

Marvel Legends Iceman

Marvel Legends Deadpool

Marvel Legends Nico Minoru

Marvel Legends Angela (Aldrif Odinsdottir)

Marvel Legends Shatterstar

Marvel Legends Lady Loki

Marvel Legends Psylocke

Marvel Legends Magik (never depicted as any orientation in canon, but has once been depicted in a relationship with another woman in an AU story, which is the only evidence of her having been written in a romantic relationship)

Marvel Legends Valkyrie (MCU) (stated to be bisexual by both the actress and director; also used as the basis for the Exiles comic version, who is explicitly gay)

Marvel Legends Kate Bishop (never explicitly stated to be queer, but heavily implied by numerous other close friends to be somewhere on the queer spectrum)

Marvel Legends Mystique

DC Universe Classics Captain Cold (kinda unsure about this one, but in the Arrowverse he's played by an openly gay actor, and his alternate universe version is in a relationship with The Ray... so maybe)

DC Multiverse Batwoman

DC Multiverse Wonder Woman (might also use the DCUC version for a separate set, but the DCM version is from the Rebirth-era, which specifically calls Diana out as being bisexual/pansexual)

DC Multiverse The Ray (Ray Terrill)

DC Classics Young Justice Aqualad (Kaldur'ahm) (stated by the creators to be on the queer spectrum; comics counterpart is explicitly gay)

Overwatch Ultimates Tracer

oop, forgot a DC one

DC Icons Harley Quinn (also may use the modern DCM Rebirth version, we'll see)

Overwatch Ultimates Sombra (need to double-check this, since she's not stated to be any orientation in the game, but translations of easter eggs in the Havana map seem to imply that she's into writing fan-fiction, with a self-insert being the object of a woman's affections in the story)

Transformers Chromedome and Rewind

Transformers Cyclonus and Tailgate

Transformers Dustup and Jumpstream

Transformers Prime Knock Out and Breakdown (not explicitly stated in the series, but very strongly implied; in comics, they're an explicitly married couple)

Transformers Brainstorm

Transformers Arcee (both a transwoman and a lesbian)

Transformers Energon Mirage

Transformers Flowspade (may or may not do her and Megaempress, since it's not entirely clear if Flowspade's affection is one-sided or mutual)

Transformers Overlord (still considering, but: Japanese-continuity has Overlord consist of a husband-and-wife duo who take turns controlling the body, putting into question what gender Overlord is; IDW continuity had Overlord take on the guise of 'Megan Guiglione', a human woman; and at least one passing reference is made to a version that is genderfluid

Transformers Prowl (implied to have been in a relationship with Chromedome in the past)

Transformers Alpha Bravo and Offroad (not explicitly stated, but their relationship in the FunPub stories is practically dripping with romantic subtext and not-so-subtext)

Transformers Cyberverse Acid Storm (genderfluid)

Transformers Cindersaur (pack-in cards with the POTP release have alternately used male and female pronouns, which Hasbro has not made a statement on either being an error; additionally, the toy is a repaint of a female-patterned toy, as a traditionally male-patterned character)

Beast Wars Airazor and Tigatron (Airazor was dubbed as a male in Japan, making their relationship homosexual; additionally, Tigatron's tiger mode was scanned off of his partner, a female tiger named Snowstalker, despite being a male-patterned robot)

Beast Wars Neo Archadis (on top of being depicted with many stereotypically 'gay' traits because Japan, Archadis is a transman from the same continuity as the genderfluid Overlord mentioned above)

Robots in Disguise (2001) Gas Skunk (in Japan, Gas Skunk's speech patterns and mannerisms are decidedly effiminate, and in later Transformers 4koma, he's depicted as working at a 'little brother' maid cafe for gay clientele)

Pffft I think you’ve covered just about anything I could think of, wow